Effects of pretreating platelets with various inhibitors on FKN-mediated enhanced adhesion. Washed platelets were preincubated with vehicle alone (untreated), a neutralizing anti-CX3CR1 antibody, isotype-matched control (control IgG), the anti-GPIIb/IIIa antibody C7E3 (anti-IIb/IIIa), or the anti-GPIbα antibody HIP-1 as indicated for 30 minutes before reconstitution with autologous RBCs and perfused over VWF and FKN coimmobilized with VWF. (A-B) Perfusion was at a wall shear rate of 150 s⁻¹ (A) and 600 s⁻¹ (B). Shown is the percentage of platelets adhering to FKN coimmobilized with VWF, and the number of platelets adhering to VWF alone (C-D) was set to 100% for each treatment (dotted line). *P < .05, **P < .01, ***P < .001 in one sample t test versus 100%, which represents the adhesion rate of platelets to VWF only surfaces. (C-D) The absolute number of platelets adhering to VWF-coated surface at the wall shear rate of 150 s⁻¹ (C) and 600 s⁻¹ (D). Results are the mean ± SEM of at least 4 independent experiments with blood from different donors.