Figure 3
![Figure 3. Direct effect of NAADP on [Ca2+]i mobilization. (A) Representative plot of NAADP-AM–induced Ca2+ mobilization in EA.hy926 cells. (B) Increase in F/F0 in cells stimulated with NAADP-AM compared with cells stimulated with vehicle alone (control). Cells were stimulated with 100μM TMPH after microinjection with (C) vehicle alone or (D) inactivating concentrations of NAADP. Increase in F/F0 in response to TMPH (E) and 2 U/mL of thrombin (F) in cells microinjected with the inactivating concentration of 500μM NAADP compared with cells microinjected with vehicle alone. Where applicable, error bars represent SEM; n = 3 independent experiments; *P < .05 by Student t test.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/117/18/10.1182_blood-2010-02-266338/4/zh89991169790003.jpeg?Expires=1735833299&Signature=ln--75-veaNMbRuxGFnPPo53xg45mK4OxuTxcZZJkwYpDwY783TUy3zvWbgbiHgVgam8t8mcik-bIqIWKfO9hTuElSXXxU7WNeNP5us6cf5lpVTvwll9M0uyakP5A2Ibj6~P8Mc1xBKhsS36p78lK9LJePHw09ADW4-6IMkiLuGGIHZtozBEEBDQuPauqp9JgEq5XPlwu0yxUGZBEra7fz2~M7FbuX-BlLClStGqlvkuzDlHko5bf9cMvSZUcHuDMMkdDMXOUf3X534VhEsk2IJxRSMnOMpNq1-xnxTlTirYkf6nkLOdxkVQ7f3wz-csWGIPhsocwxAcseBNaIBClg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Direct effect of NAADP on [Ca2+]i mobilization. (A) Representative plot of NAADP-AM–induced Ca2+ mobilization in EA.hy926 cells. (B) Increase in F/F0 in cells stimulated with NAADP-AM compared with cells stimulated with vehicle alone (control). Cells were stimulated with 100μM TMPH after microinjection with (C) vehicle alone or (D) inactivating concentrations of NAADP. Increase in F/F0 in response to TMPH (E) and 2 U/mL of thrombin (F) in cells microinjected with the inactivating concentration of 500μM NAADP compared with cells microinjected with vehicle alone. Where applicable, error bars represent SEM; n = 3 independent experiments; *P < .05 by Student t test.
