Expression analysis in LSK cells of gene-corrected mice. (A) Average Mpl expression in LSK cells sorted from Mpl^−/− mice, which were transplanted with Mpl^−/− cells transduced with the vector as indicated, determined by quantitative RT-PCR (mean ± SEM). LSK cells from 3 Mpl^−/− mice transplanted with wt lin⁻ BM were used as reference. (B) Fold difference of expression of genes as indicated in LSK cells of Mpl-corrected Mpl^−/− mice compared with eGFP expressing Mpl^−/− LSK cells. RNA was isolated form 3 Mpl-corrected mice and 3 eGFP control mice. Expression was determined by microarray analysis. Black bars represent the genes that were regulated with P < .05 (t test with Bayesian shrinkage). Array results and results of validating quantitative RT-PCR are shown in supplemental Figures 9 and 10.