Ndfip1^−/− mice show increased iron uptake under low iron conditions and increased iron storage under normal iron conditions. (A) Serum iron levels and (B) transferrin saturation (Tf sat) in Ndfip1^+/+ and Ndfip1^−/− mice fed a low- or normal iron diet. Both serum iron and transferrin saturation are increased in Ndfip1^−/− mice on the low-iron diet compared with Ndfip1^+/+ mice. Iron stores in (C) liver and (D) spleen. Data represent mean ± SD, *P < .05, n = 3-7. Bone marrow iron levels in (E) Ndfip1^+/+ mice and (F) Ndfip1^−/− mice on the low-iron diet and in (G) Ndfip1^+/+ mice and (H) Ndfip1^−/− mice on the normal diet. Scale bar, 50 μm. Iron stores are significantly increased in Ndfip1^−/− mice on the normal diet compared with Ndfip1^+/+ mice.