Ndfip1^−/− mice fed a low-iron diet develop hypochromic, microcytic anemia. (A) Hemoglobin (Hb), (B) hematocrit (Hct), and (C) RBC count are decreased in Ndfip1^−/− mice compared with Ndfip1^+/+ mice. Blood smears from (D) Ndfip1^+/+ mice and (E) Ndfip1^−/− mice show RBCs from Ndfip1^−/− mice are microcytic and hypochromic compared with their wild-type counterparts. Scale bar, 50 μm. (F) Erythroid, lymphoid, and myeloid series as a percentage of total cells in the bone marrow smears. (G) The myeloid to erythroid ratio in bone marrow. The percentage of erythroid series cells is decreased in Ndfip1^−/− mice, and the myeloid to erythroid ratio is increased, indicating impaired erythropoiesis. Data represent mean ± SD, *P < .05, n = 4-7.