Removing inflammation reveals further evidence for the importance of Ndfip1 in the regulation of DMT1 in vivo. (A) Hb, (B) Hct, and (C) RBC count are significantly increased in Ndfip1^−/−/Rag1^−/− mice compared with single knockout Ndfip1^+/+/Rag1^−/− mice and Ndfip1^−/−/Rag1^+/+ and are restored to Ndfip1^+/+/Rag1^+/+ levels. (D) Serum iron and (E) transferrin saturation in (Ndfip1^+/+/Rag1^+/+) as for other genotypes Ndfip1^−/−/Rag1^+/+, Ndfip1^+/+/Rag1^−/−, and Ndfip1^−/−/Rag1^−/− mice. Both serum iron and transferrin saturation are elevated in the double knockout mice. DMT1 expression in the duodenum of (F) Ndfip1^+/+/Rag1^−/− mice, (G) Ndfip1^−/−/Rag1^+/+ mice, and (H) Ndfip1^−/−/Rag1^−/− mice. DMT1 levels are increased in mice lacking Ndfip1 (Ndfip1^−/−/Rag1^+/+ and Ndfip1^−/−/Rag1^−/− mice) compared with Ndfip1^+/+/Rag1^−/− controls. All mice were fed a low-iron diet for 3 weeks, data represent mean ± SD, *P < .05, n = 3-4.