Comparison of integrin activation in WT and kindlin-2^+/− aortic endothelial cells. (A) Dose-dependence of soluble Alexa 488–labeled Fg binding to resting or PMA-stimulated ECs. Soluble Fg binding to kindlin-2^+/− ECs is decreased and independent on PMA stimulation. (B) Soluble Fg (50 μg/mL) binding to WT and kindlin-2^+/− ECs in the presence of VEGF and Mn²⁺ ions. Fg binding to kindlin-2^+/− ECs is significantly reduced and independent on VEGF stimulation. (C) Soluble FITC-collagen I (50 μg/mL) binding to WT and kindlin-2^+/− ECs in the presence of VEGF and Mn²⁺ ions. Collagen I binding to WT and kindlin-2^+/− ECs is the same in the absence or presence of VEGF. All data are means ± SEM of triplicate samples and are representative of 3 independent experiments with pooled ECs from 4-6 mice.