The FasL ICD regulates expression of proliferation-associated and Wnt signaling target genes. Differential expression of selected genes, which represent direct targets of the Wnt signaling pathway and/or that are involved in lymphocyte activation and proliferation, is shown in wild-type and FasL ΔIntra B cells. Gene expression in both cell populations was quantified by real-time PCR with cDNA derived from stimulated splenic B cells (1 μg/mL anti-IgM antibody for 4 hours). Each sample was assessed in triplicate and normalized to the housekeeping gene Hprt. The relative change of gene expression in FasL ΔIntra cells was calculated with the comparative threshold cycle method (2^−ΔΔCt). The columns for each gene represent the mean of 4 independent experiments, and the black bars indicate SEs.