Effect of HIF-1α loss of function on BMDAC survival and gene expression. (A-C) Bone marrow cells were isolated from Hif1a^+/− (HET) mice, which are heterozygous for a null (knockout) allele at the locus encoding HIF-1α, or from their wild-type (WT) littermates and cultured for 4 days under nonhypoxic (N) or hypoxic (H) conditions in endothelial growth medium. The percentage of viable cells (A), number of viable cells (B), and expression of HIF-1 target genes (C) were determined. *P < .05 compared with nonhypoxic WT cells. #P < .01 compared with hypoxic WT cells in panels B and C, Bonferroni post hoc comparisons after 1-way ANOVA. (D) Quantitative RT-PCR analysis of HIF-1 target gene expression and cell viability (E) in BMDAC-V and BMDAC-D cultured for 4 days in the presence (+) or absence (−) of 100nM digoxin (Dig). *P < .05 compared with BMDAC-V without digoxin. #P < .01 compared with BMDAC-D without digoxin, Bonferroni post hoc comparisons after 1-way ANOVA. Data are mean ± SEM (n = 3 or 4).