DMOG increases BMDAC survival in ischemic limbs. Genomic DNA was isolated from ischemic and nonischemic gastrocnemius muscles of female mice subjected to unilateral femoral artery ligation. All mice were injected with 2 × 108 pfu of AdCA5 distributed equally at 4 intramuscular injection sites in the ischemic limb immediately after surgery. A total of 5 × 105 BMDACs (isolated from male mice and cultured in vehicle or DMOG) were injected intravenously 24 hours after surgery. Muscle was harvested at 8, 16, 34, and 58 hours after injection. DNA was isolated and analyzed using quantitative PCR for a Y-chromosome-specific Sry gene sequence. No signal was detected in gastrocnemius muscles from nonischemic limbs. Decay curves were constructed using a 1-phase exponential decay model with nonlinear regression. BMDAC half-life was calculated from the data, and the difference between vehicle and DMOG was statistically significant (P < .05). Two-way ANOVA showed a statistically significant overall effect of DMOG treatment on BMDAC survival (P < .01 n = 3 or 4 animals per time point). Data are mean ± SEM.
