Figure 3
Figure 3. Effect of SIRT1 deletion on in vitro vascular sprout formation in differentiating EBs. (A) Representative micrographs of 3-dimensional in vitro angiogenesis assays with collagen gel-embedded spheroids generated from WT (SIRT1+/+) or KO (SIRT1−/−) day 6 EBs. (B) Analysis of the percentage of angiogenic EBs was performed after 8 days of secondary culture in collagen gel. (C) Representative micrographs of in vitro matrigel assays with SIRT1+/+ or SIRT1−/− day 6 EBs. (D) Percentage of each class of vascular sprouting (see “Vascular sprout formation assay”). Data are shown as mean ± SEM. N = 3; *P = .01-.04. Image acquisition details: Nikon Diaphot microscope, 10×/0.25 numeric aperature objective lens, Nikon F3 camera, NIS-Elements D2.30 software.

Effect of SIRT1 deletion on in vitro vascular sprout formation in differentiating EBs. (A) Representative micrographs of 3-dimensional in vitro angiogenesis assays with collagen gel-embedded spheroids generated from WT (SIRT1+/+) or KO (SIRT1−/−) day 6 EBs. (B) Analysis of the percentage of angiogenic EBs was performed after 8 days of secondary culture in collagen gel. (C) Representative micrographs of in vitro matrigel assays with SIRT1+/+ or SIRT1−/− day 6 EBs. (D) Percentage of each class of vascular sprouting (see “Vascular sprout formation assay”). Data are shown as mean ± SEM. N = 3; *P = .01-.04. Image acquisition details: Nikon Diaphot microscope, 10×/0.25 numeric aperature objective lens, Nikon F3 camera, NIS-Elements D2.30 software.

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