Figure 6
Figure 6. Ectopic expression of exogenous SIRT1 in SIRT1−/− ESCs. (A) Diagram of pLenti6-SIRT1-Venus lentiviral vector. (B) Transfected SIRT1−/− ESCs were sorted, and expression of SIRT1 protein was determined by Western blotting. (C) Number of primitive erythroid colonies generated by day 4.5 and day 5 SIRT1+/+, SIRT1−/− ESCs. (D-E) qRT-PCR analysis of mRNA levels of embryonic globin (Hbb-bh1) and adult globin (Hbb-b1) in day (D) 0-8 SIRT1+/+, SIRT1−/− plus null and SIRT1−/− +SIRT1 venus EBs. Data represent fold changes in EBs, relative to SIRT1+/+ D0 control and comparison between SIRT1+/+, SIRT1−/− plus null, and SIRT1−/− plus SIRT1 EBs at each time point. Results are average of 3 independent experiments. Each data point denotes 6 biological replicates; *P < .05; **P < .01; ***P < .001.

Ectopic expression of exogenous SIRT1 in SIRT1−/− ESCs. (A) Diagram of pLenti6-SIRT1-Venus lentiviral vector. (B) Transfected SIRT1−/− ESCs were sorted, and expression of SIRT1 protein was determined by Western blotting. (C) Number of primitive erythroid colonies generated by day 4.5 and day 5 SIRT1+/+, SIRT1−/− ESCs. (D-E) qRT-PCR analysis of mRNA levels of embryonic globin (Hbb-bh1) and adult globin (Hbb-b1) in day (D) 0-8 SIRT1+/+, SIRT1−/− plus null and SIRT1−/− +SIRT1 venus EBs. Data represent fold changes in EBs, relative to SIRT1+/+ D0 control and comparison between SIRT1+/+, SIRT1−/− plus null, and SIRT1−/− plus SIRT1 EBs at each time point. Results are average of 3 independent experiments. Each data point denotes 6 biological replicates; *P < .05; **P < .01; ***P < .001.

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