Zeb2 is highly expressed in embryonic hematopoietic clusters and adult HSCs. (A) qRT-PCR using Zeb2-specific primers on total RNA samples prepared from peripheral blood mononuclear cells (PBMC), bone marrow (BM), FACS-sorted hematopoietic progenitor cells (MEP, megakaryocyte/erythroid progenitors; GMP, granulocyte/monocyte progenitors; CMP, common myeloid progenitors), and HSCs from adult mice. As a negative control, RNA from feeder-free Zeb2-null ES cells and normal mammary epithelial (Nme) mouse cells were used. As a positive control for Zeb2 mRNA expression, RNA from mouse embryonic fibroblasts (MEF) was used. Bars represent mean ± SD. Two independent sorting experiments were performed and qRT-PCR was done in triplicate on biological duplicates (B) Zeb2 immunohistochemical staining of sections of the AGM region of a E10.5 embryo (400× magnification), demonstrating Zeb2 protein presence in hematopoietic clusters (arrows) budding from the hemogenic endothelium of the dorsal aorta.