Hematopoietic-specific loss of Zeb2 decreases Ang1 expression in the head and is associated with decreased desmin⁺ pericyte coverage of the head vasculature. (A) qRT-PCR for angiopoietin-1 on RNA isolated from wild-type and Zeb2^{−/ΔTie2-Cre} heads. Technical (n > 3) and biological replicates (n > 5 embryos per genotype) were used. Bars represent mean ± SD; *P < .05; **P < .01; ***P < .001. (B) CD31/desmin immunofluorescence staining on Zeb2^{−/ΔVav-iCre} and control wild-type head vasculature showing lack of desmin⁺ cell coverage of the head vasculature in Zeb2^{−/ΔVav-iCre} neonates.