Functional restoration of ROS production in neutrophils differentiated from iXC1-APC-gp91 c3. Panels A and B are cytospin Giemsa stain and DHR flow cytometric analysis, respectively, of neutrophils arising from differentiation of normal control iPSC line iPS(IMR90); and panels C and D are cytospin Giemsa stain and DHR flow cytometric analysis, respectively, of neutrophils arising from differentiation of ZFN-targeted minigene-corrected X-CGD iPSC clone iXC1-APC-gp91 c3. Arrows in the cytospin Giemsa-stain images (A,C) indicate some of the cells with mature neutrophil morphology (bar = 10 μm). The DHR dot plot images (y-axis side scatter; x-axis DHR fluorescence) demonstrate robust ROS production (high fluorescence) by 28.8% and from 26.8% of cells, respectively, representing PMA-activated neutrophils derived from normal control iPSCs (B) and from ZFN-targeted minigene-corrected X-CGD iPSC clone iXC1-APC-gp91 c3 (D).