Effects of ERG expression on T-cell repopulation. (A-B) Thymocytes taken from mice that received a transplant with GFP-only control and ERG-transduced BM cells 5 weeks earlier were each transplanted (9 × 106 per mouse) into 3 lethally irradiated syngeneic hosts along with 3 × 105 radioprotective BM cells. Mice were then analyzed for GFP expression in thymus 5 weeks (1 mouse) and 9 weeks (2 mice) after the transplantation. A mouse indicated with an asterisk exhibited Notch1 mutation in thymocytes, whereas all other mice had no detectable Notch1 mutations. (C) Microarray analysis of transcripts in thymocytes taken from mice that received a transplant with GFP-only control and ERG-transduced BM cells 5 weeks earlier. Gene Set Enrichment Analysis showing enrichment of genes associated with cell-cycle and E2F1 target genes. Notch1 mutations were not detected in this experiment.
