Dex-induced protein synthesis is not required for inhibition of CD95L mRNA expression. (A) Primary murine day-5 T cells were restimulated by anti-CD3 antibody in the presence or absence of 10⁻⁷ M Dex for the indicated periods of time. The mRNA expression level of CD95L and β-actin was detected by RT-PCR. (B) Primary murine day-5 T cells were restimulated by anti-CD3 antibody in the presence or absence of 10⁻⁷ M Dex for 5 hours. The mRNA expression of CD95L, GILZ, Bcl-2, and β-actin was detected by RT-PCR. (C) Same as in panel B, but restimulation with or without pretreatment for 2 hours with 10 μg/mL CHX. The mRNA expression of CD95L and β-actin was detected by RT-PCR. Results are representative of at least 3 independent experiments.