Retrovirus-mediated SATB1 gene transduction confers growth disadvantage to Hut78 cells. (A) Immunofluorescence analysis show that Hut78 cells transduced with SATB1 gene in retrovirus vector (Hut78-SATB1) stably express SATB1 protein in cell nucleus, whereas control cells transduced with empty vector (Hut78-MIG) do not express SATB1. Original magnification ×630. Scale bar represents 10 μm. (B) Parental Hut78 cells (black) and Hut78-MIG cell clones (navy) present growth advantage over SATB1-transduced cell clones (light blue). On day 4, the viable cell numbers of Hut78-SATB1 cell clones were significantly lower than those of parental Hut78 cells and Hut78-MIG cells. Data were generated from the individual cell clones from each group, and all experiments were triplicated. *P < .05 by Mann-Whitney U test.