Cell-cycle and apoptosis analysis by flow cytometry. SATB1-transduced and control cell clones were analyzed in propidium iodide cell-cycle analysis (A) and annexin V binding-based apoptosis assay (B). (A) Each cell cycle is composed of G₁, S, G₂ stages, as well as sub-G₁ stage, which represents the apoptotic or dead population of cells. Proportions of sub-G₁ stage are indicated as no. (%). Chart on right: SATB1-transduced cells revealed increased sub-G₁ population in contrast to the control cells. (B) Proportions of annexin V⁺ population, which represents apoptotic and dead cells are indicated as no. (%). Chart on right: SATB1-transduced cells revealed increased annexin V population in contrast to the control cells. Hut78 indicates parental untransduced Hut78 cells; MIG-1 to MIG-3, Hut78 cell clones transduced with empty MIG vector; and SATB1-1 to SATB1 4, Hut78 cell clones transduced with MIG vector containing SATB1. *P < .05 by Mann-Whitney U test.