Vav1 and Vav3 may play redundant but critical roles in in vivo thrombus formation in mice fed high-fat diet. (A) Time to thrombotic occlusion of carotid arteries from vav1^−/− mice and their age-matched wild-type mice fed high-fat diet for 2 weeks was measured after 1 minute topical application of 7.5% FeCl₃. Platelets were labeled by direct injection of fluorescence dye Rhodamine 6G into the jugular vein. Thrombi formation in the carotid artery was assessed under intravital microscopy. (B) Time to thrombotic occlusion of carotid arteries from vav1^−/−;vav3^−/− and their age-matched wild-type mice fed high-fat diet for 4 weeks was measured after 1-minute topical application of 7.5% FeCl₃.