Response of peptide-specific T-cell clones to endogenously processed antigen presented by DCs. (A) T-cell clones from different donors specific for the peptides p1, p22, p27, p41, p48, p59, p69, and p84 were stimulated with peptide-pulsed DCs or (B) DCs transfected with Crf1 mRNA (n = 3). Number of T-cell clones reacting to Crf1 mRNA-transfected DCs/number of peptide-specific T-cell clones: p1 donor 5, 3/3; p1 donor 6, 1/5; p1 donor 7, 0/6; p1 donor 12, 10/10; p22 donor 5, 1/2; p27 donor 7, 1/3; p41 donor 1, 11/11; p41 donor 2, 16/16; p41 donor 4, 2/2; p41 donor 7, 4/4; p41 donor 10, 3/3; p48 donor 3, 0/7; p48 donor 4, 0/4; p48 donor 11, 0/5; p59 donor 5, 1/3; p59 donor 6, 2/2; p59 donor 12, 1/7; p69 donor 1, 0/3; p69 donor 4, 0/2; p84 donor 11, 0/5; p84 donor 4, 0/4. Shown is 1 clone per antigen and donor.