Karyograms of BM cells from a patient with CML and the 2 iPSCs derived from these cells. Top left panel shows spectral karyogram of CML iPSC15. SKY analysis demonstrates the 4-way translocation between chromosomes 1, 9, 11, and 22, shown here by classification-colored metaphase chromosomes. Translocations are apparent by the different colors of translocated segments representing the chromosome of origin. The Philadelphia chromosome is indicated by the red arrow. Standard G-banded karyotyping (top right and bottom panels) shows the complex 4-way translocation t(1;9;22;11)(p34.1;q34;q11.2;q23) found in all cells examined from the BM and from both iPSC lines (CML iPSC). The translocation 9;22 breakpoints of the BCR/ABL fusion are embedded in this rearrangement.