In vitro effects of CXCL12 on generation of NK cells from LMPPs or iNKs. (A) Sorted Lin⁻Sca-1⁺c-kit⁺ (LSK) Flt3^hi LMPPs were cultured in medium containing Flt3L, SCF, and IL-15 in the absence or presence of CXCL12. After 14-day culture, CD3⁻NK1.1⁺ NK cells were counted by flow cytometry; n = 4. *P < .05. (B) Sorted CD3⁻NK1.1⁺DX5⁻ iNKs were cultured in medium containing IL-15 in the absence or presence of CXCL12. After 3-day culture, the number of CD3⁻NK1.1⁺DX5⁺ mNKs were measured by flow cytometry; n = 4. *P < .05.