Comparison between Lhx2 and Hoxb4. (A) Retroviral vectors. (B-C) Frequencies of KSL cells on day 15 (B) and between day 12 and day 20 (C). The data were shown as mean ± SD (n = 6). Asterisks indicate significant differences (P < .01 by t test). (D-E) The RT-PCR analysis of Lhx2 and Hoxb4 expression (D), and Hoxb4 target genes (E). (F) Verification of the separation of EGFP/EYFP fluorescence. (G) KSL frequency of Lhx2 (EYFP⁻/EGFP⁺)–, Hoxb4 (EYFP⁺/EGFP⁻)–transduced and cotransduced (EYFP⁺/EGFP⁺) cells. Cells within Lin⁻ compartments were shown in bottom panels.