Composition and differentiation of Lhx2-transduced hematopoietic cells. (A) FACS analysis of lineage-restricted HPCs derived from ESCs. (B-C) Morphology of representative colonies (B) and colony forming units per 104 cells (C) of uninfected and Lhx2-transduced cells derived from ESCs. Original magnification: ×4. The data were shown as mean ± SD (n = 5). (D-E) FACS analysis (D) and May-Grünwald-Giemsa staining (E) of the cells differentiated from Lhx2-transduced KSL cells derived from ESCs. Original magnification: ×40. (F) Effects of Lhx2 on erythroid production from ESCs and on β-globin expression.