Functional analysis of the Lhx2-transduced donor cells derived from ESCs. (A) Study design. (B) Cell sorting of the donor cells from the primary recipients. (C) Colony assays. EGFP⁺ cells in were cultured in semisolid media for 1 week. Representative colony was shown. The colony numbers per 2000 cells are shown as dots. The bar indicates the mean value. (D) Frequency of donor cells in peripheral blood of secondary recipients. (E) FACS analysis of bone marrow of secondary recipients. Representative data were shown.