Transplantation analysis of iPSC-derived hematopoietic cells amplified by Lhx2. (A) Study design. (B and C) FACS analysis at 6 weeks after transplantation of iPSC-derived hematopoietic cells. (D-F) Long-term hematopoiesis repopulation by Lhx2-transduced hematopoietic cells derived from iPSCs (clone 24). (G) FACS analysis of peripheral blood of recipients transplanted with iPSC-derived cells. (H) May-Grünwald-Giemsa staining EGFP⁺/Ter-119⁺ cells and EGFP⁺/Gr-1⁺ cells. Original magnification: ×30. (I) Rearrangement of immunoglobulin gene loci in iPSC-derived B-lymphoid cells. EGFP⁺/B220⁺ cells were sorted from recipient mice transplanted with iPSC-derived hematopoietic cells and analyzed. (J) Long-term monitoring of chimerism in the peripheral blood of recipient mice transplanted with Lhx2-transduced hematopoietic cells derived from iPSC clone 24, clone 2, and RENKA ESCs.