Expression of Lhx2 in vivo. (A) Expression of Lhx2 mRNA in the donor-derived differentiated cells. The donor-derived erythroid, B-lymphoid and progenitor cells were purified from a recipient transplanted with ESC-derived cells and subjected to RT-PCR analyses. (B) The Lhx2 expression in normal mouse. BM, SP, and TH indicate bone marrow, spleen, and thymus, respectively. Head region dissected from E12.5 mouse embryos was a positive control. (C) Western blot of FLAG.Lhx2 expression in donor-derived differentiated cells. ESC-derived HSC-like cells were amplified by Flag.Lhx2 and transplanted. The donor-derived Mac-1⁺ cells and control Mac-1⁺ cells were purified and analyzed.