IL-22R1 tg mice exhibit neutrophilia. (A) Forward scatter/side scatter plot shows that the percentages of GR1⁺CD11b⁺ population was higher in IL-22R1 tg mice compared with the wt mice. The Gr1⁺CD11b⁺ population was further distinguished based on the FSC and F4/80 gates. (B) Percentage of neutrophils (FSC^hiF4/80^lo) and monocytes (FSC ^loF4/80^int) from polymorphonuclear leukocytes. (C-D) Percentage of cells that are Gr1⁺CD11b⁺ in bone marrow and spleen (n = 3), respectively. (E-F) Representative cytospins of 1 × 10⁴ cells from bone marrow (×20; E) and spleen (×20; F) stained with Giemsa. Images were acquired with an Olympus BX40 light microscope using Image Proplus 6 software.