Changes in proportion and absolute number of MZ B cells in CD19:KLF3 and KLF3-deficient mice. (A) Spleen follicular (CD21/35− CD23+) and MZ B-cell (CD21/35hi CD23−) proportions in normal and CD19:KLF3 transgenic lines (founder line 2, 23, and double-transgenic mice). Cells were stained for B220, CD21/35, and CD23. Data shown are gated on B220+ cells. Total numbers of B220+ cells are indicated above the plots (± SD). (B) Numbers of the indicated subsets were determined among CD19:KLF3 and nontransgenic littermates of founder lines 2 and 23 at 6 to 7 or 9 to 12 weeks of age, respectively, as shown. (C) Immunofluorescent analysis of spleen cryosections of CD19:KLF3 and littermate control mice stained for Moma-1 and IgM (R33-24-12). (D) Splenic B cells from normal (FVB/NJ) and KLF3-deficient (FVB/NJ.KLF3d/d) mice at 12 weeks of age. Data shown are gated on B220+ cells. (E) MZ B-cell numbers in mice as shown in panel D (n = 8 each). Double-transgenic mice were identified by Southern blot (A, n = 2 each). (B-E) Each dot represents an individual animal. (C) Immunohistologic analysis has been performed on more than 5 mice each of CD19:KLF3 and normal genotype. Micrographs were obtained with Axioscope instrumentation, using a 5× objective.
