c-Met regulates progenitor cell egress by ROS generation. (A) A representative FACS plot showing ROS levels on BM SKL cells of mice receiving vehicle or G-CSF with vehicle or c-Met inhibitor. (B) A representative FACS analysis of ROS levels on BM SKL of control mice or mice injected for 2 consecutive days with HGF. (C) ROS levels in circulating SKL cells in mice receiving 2 injections of HGF or 5 consecutive injections of G-CSF together with DMSO or c-Met inhibitor. (D) Circulating leukocytes in mice receiving 2 consecutive injections of NAC, HGF alone, or together with NAC or (E) in mice injected for 5 days with G-CSF alone or NAC on days 4 and 5 of G-CSF treatment. (F-G) Colony-forming progenitor cells in the blood of mice that were given NAC, HGF, HGF together with NAC (F) or G-CSF alone or in combination with NAC (G). (H) A representative FACS analysis of circulating SKL cells in mice injected with NAC, G-CSF alone or with NAC, or HGF alone or in association to NAC. (I) A summary of circulating SKL in mice receiving HGF alone or in combination with NAC. (J) A summary of SKL in the circulation of mice receiving NAC, G-CSF, or G-CSF and NAC. (K) SDF-1 induced migration of BM cells derived from control mice or mice treated with G-CSF alone or in combination with NAC. (L) ROS levels in circulating SKL cells in mice receiving 5 daily injections of G-CSF alone or in combination with 2 consecutive injections of NAC. Indicated values represent the mean ± SE in ≥ 3 independent experiments, 6 mice in each group. **P < .001; *P < .05.