Expression of Kdm2b/Jhdm1b is up-regulated in leukemic stem cells. (A) KDM2B/JHDM1B is overexpressed at the RNA level in human AML, T-cell acute lymphoid leukemia (T-ALL), and B-cell acute lymphoid leukemia (B-ALL) compared with their normal controls. Data are derived from Oncomine. ²²  (B) Kdm2b/Jhdm1b is overexpressed in Hoxa9/Meis1-induced AML samples compared with that of normal BM. Relative mRNA levels are measured by RT-qPCR and normalized to Gapdh level. The level in normal BM is arbitrarily set to 1. (C) Separation of Hoxa9/Meis1-transformed murine AML cells into c-kit^high and c-kit^low populations by FACS. These 2 cell populations are used for methylcellulose colony formation assays. (D) The c-kit^high cell population has higher colony formation capability compared with the c-kit^low population. (E) RT-qPCR analysis shows that the c-kit^high cell population expresses a higher level of Kdm2b/Jhdm1b than the c-kit^low cell population. Relative mRNA levels are measured by RT-qPCR and normalized to Gapdh level. The level of c-kit^low population is arbitrarily set to 1. FSC indicates forward scatter.