American Society of Hematology

Figure 3

$Figure 3. Cxcl12 acts as an environmental factor regulating the HSPC homeostasis. (A) Representative figures of cobblestone area formation cells (CAFCs) (arrow) derived from Cxcl12-proficient EGFP+Lin−Sca-1+ BM cells cultured on either Cxcl12-WT or Cxcl12-cKO stromal layer. Scale bar = 100 μm. Right panel is the statistics of CAFCs readout per 2 × 103 input cells per well (mean ± SEM; **P < .005; n = 3). (B) LTC-IC assay in which equal numbers of Cxcl12-proficient BM test cells were seeded on either Cxcl12-WT or Cxcl12-cKO stromal cell layers. The horizontal bars indicate the mean percentage (**P < .005; n = 7). (C) Left panel is the schematic drawing of experimental strategy. The bar graph on the right panel shows the percentage of CD45.1+cKit+Sca-1+ cells in Lin− fraction in recipients' BM and PB (mean ± SEM; *P < .05; n = 4).$

Cxcl12 acts as an environmental factor regulating the HSPC homeostasis. (A) Representative figures of cobblestone area formation cells (CAFCs) (arrow) derived from Cxcl12-proficient EGFP⁺Lin⁻Sca-1⁺ BM cells cultured on either Cxcl12-WT or Cxcl12-cKO stromal layer. Scale bar = 100 μm. Right panel is the statistics of CAFCs readout per 2 × 10³ input cells per well (mean ± SEM; **P < .005; n = 3). (B) LTC-IC assay in which equal numbers of Cxcl12-proficient BM test cells were seeded on either Cxcl12-WT or Cxcl12-cKO stromal cell layers. The horizontal bars indicate the mean percentage (**P < .005; n = 7). (C) Left panel is the schematic drawing of experimental strategy. The bar graph on the right panel shows the percentage of CD45.1⁺cKit⁺Sca-1⁺ cells in Lin⁻ fraction in recipients' BM and PB (mean ± SEM; *P < .05; n = 4).

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