Figure 3
![Figure 3. Effects of PGE1 on platelet activation and fibrin formation. Platelets were superfused with plasma at 250 s−1 in the absence (control) or presence (0.5μM) of PGE1. Bright-field and contrast images (Nikon Diaphot 200 microscope, see “Methods”) were taken at 0-60 seconds after start of fibrin formation (bars, 10 μm). (A) Reduced fibrin on platelets after PGE1 treatment (average pixel intensities of subtracted images). (B) Lower [Ca2+]i rises with PGE1 in Fluo-4–loaded platelets. (C) Lower OG488–annexin A5 staining with PGE1. (D) Reduced OG488-fibrin(ogen) staining (integrated fluorescent intensities). Means ± SE; n ≥ 3; *P < .05, ***P < .001 vs control.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/117/2/10.1182_blood-2010-01-262683/4/zh89991064420003.jpeg?Expires=1735569908&Signature=UHfemUUrcTFjkyV1cuUHC1551GEwwJeEj-4WVPuH1AVF4ONA1toe9zRw~QB0A-cOEkHjEMrLSagyPTdOoMgTjRUMy7BvTGZfuefKitG2mF37tIeTeURhn9qGWUWXXR80xP7Qfa7beSbvN09SpWhQKlGPoc9pYvEhTzlxq~WsVqcpQL1FWyMqo2QihhVLeen~ubSiIhTxisT4Q1NOLlz-Ebu232Q1Hd~wKIIQ201vsa7It4PS-FPLZ-qTLVozGJFA3-H0rKwETw5unJGFylAdq2Iefrfrtw9UA71eQKp4C0cnt67z0MreELBRnqYfw8g3zAnEP4C70HRBBm44k9J-Sw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of PGE1 on platelet activation and fibrin formation. Platelets were superfused with plasma at 250 s−1 in the absence (control) or presence (0.5μM) of PGE1. Bright-field and contrast images (Nikon Diaphot 200 microscope, see “Methods”) were taken at 0-60 seconds after start of fibrin formation (bars, 10 μm). (A) Reduced fibrin on platelets after PGE1 treatment (average pixel intensities of subtracted images). (B) Lower [Ca2+]i rises with PGE1 in Fluo-4–loaded platelets. (C) Lower OG488–annexin A5 staining with PGE1. (D) Reduced OG488-fibrin(ogen) staining (integrated fluorescent intensities). Means ± SE; n ≥ 3; *P < .05, ***P < .001 vs control.
