Expression of dPH/R364E/dC in HSCs/HPCs facilitates the repopulation of blood cells in irradiated hosts. (A) Transduction efficiencies of retroviral vectors into Lin^- cells. The percentage of eGFP-positive cells in Lin^- populations transduced with control vector (left), vectors encoding Lnk (middle), or dPH/RE/dC (right) are indicated. (B) Lethally irradiated mice received transplants with transduced Lin^- cells. Peripheral-blood cells harvested from mice 11 weeks after transplantation were stained with anti-B220, anti-CD3, or anti–Gr-1, and the contribution of eGFP-positive cells to B-, T-, or myeloid-lineage cells was analyzed. Numbers represent the percentage of cells that fall into the indicated boxes. Typical results of multiple experiments are shown. (C) Long-term repopulating ability of dPH/R364E/dC-transduced cells. The contribution of transduced cells to B-, T-, and myeloid-lineage cells was analyzed at indicated time points. ▵ indicate the control vector-transduced group; ▴, the dPH/R364E/dC-transduced group. Two representative results of 5 experiments are shown. ^*P < .05: significant differences between dPH/R364E/dC-transduced and control groups.