Transcription of CK genes in blood DC subsets exposed to influenza virus. (A) Total RNA was extracted from pDCs and mDCs of 6 healthy donors after sort (before), and of 6 other donors 24 hours after culture with influenza virus (after). Amplified cRNA was hybridized on Affymetrix HG-U133 chips. Gene expression was analyzed with GeneSpring 6.1 software. Each probe set was normalized with a per chip normalization to the 50th percentile, and a per gene normalization to the median of each gene. Within the most differentially expressed probe sets (P < .05 with Bonferroni multiple testing correction, or > 2 fold-change), we identified a set of 20 chemokine probes. (B) Transcription of 44 different chemokines. After per-chip normalization to the 50th percentile, the normalized intensity values of each probe set were used as a measurement of its expression. (C) Normalized expression of the most significantly transcribed chemokines (3-fold over the 50th percentile). Each bar is representative of mean normalized intensity plus or minus standard error for chemokine's gene expression in pDCs and mDCs from 6 healthy donors before infection or 6 healthy donors after infection.