Kinetics of CK expression. Blood DCs isolated from 4 donors were exposed to influenza virus. Total RNA was processed and gene expression was analyzed after per chip normalization as in Figure 1 (left). Protein expression was evaluated by multiplex cytokine analysis (Luminex; CCL2, CCL3, CCL4, CCL5, CXCL8, CXCL9, and CXCL10) or by ELISA (CXCL16, CCL19, CCL22, and CXCL13) (right). CXCL16 and CXCL4 are expressed before infection and their expression constantly decreases upon viral stimulation (A-D). CXCL1, CXCL2, CXCL3, and CXCL8 are transiently expressed between 2 and 8 hours, longer for CXCL8 (E-H). Inflammatory chemokines (CCL3, CCL4, CCL5) and interferon-dependent chemokines (CXCL9, CXCL10, CXCL11) expression is up-regulated between 2 and 8 hours and then decreases progressively (I-P). CCL19, CCL22, and CXCL13 are significantly expressed after 8 to 24 hours (Q-T). Finally, CCL2 and CCL8 are expressed only by infected mDCs (U-X).