American Society of Hematology

Figure 1.

$Figure 1. Overexpression of HES-1 reduces B-cell differentiation but does not promote T/NK-cell differentiation. (A) Flow cytometric analysis of MS-5 cocultures with cytokine Mix 2 (SCF and IL-7) for B-cell development. Percentages of B cells (CD19+HLA-DR+) and T/NK progenitor cells (CD7+cyCD3+) in the EGFP+-gated fraction of cultures initiated with LIE-, HES-1-, or ICN-transduced CD34+ cord-blood cells are indicated. Dot plots shown are representative of 5 independent experiments with cells from 5 different donors. (B) Flow cytometric analysis of MS-5 cocultures with cytokine Mix 3 (SCF, IL-2, IL-15) for NK-cell development. Percentage of NK cells (CD56+CD3-) in the EGFP+-gated fraction is indicated. Dot plots shown are representative of 5 independent experiments with cells from 5 different donors. Scatterplots show relative and absolute numbers of B cells and NK cells generated in 5 independent experiments. Each symbol represents an individual experiment. The mean of 5 experiments is represented by thick horizontal lines in each bottom panel. P values obtained with the paired Student t test are shown when significant; N.S. indicates a nonsignificant P value.$

Overexpression of HES-1 reduces B-cell differentiation but does not promote T/NK-cell differentiation. (A) Flow cytometric analysis of MS-5 cocultures with cytokine Mix 2 (SCF and IL-7) for B-cell development. Percentages of B cells (CD19⁺HLA-DR⁺) and T/NK progenitor cells (CD7⁺cyCD3⁺) in the EGFP⁺-gated fraction of cultures initiated with LIE-, HES-1-, or ICN-transduced CD34⁺ cord-blood cells are indicated. Dot plots shown are representative of 5 independent experiments with cells from 5 different donors. (B) Flow cytometric analysis of MS-5 cocultures with cytokine Mix 3 (SCF, IL-2, IL-15) for NK-cell development. Percentage of NK cells (CD56⁺CD3^-) in the EGFP⁺-gated fraction is indicated. Dot plots shown are representative of 5 independent experiments with cells from 5 different donors. Scatterplots show relative and absolute numbers of B cells and NK cells generated in 5 independent experiments. Each symbol represents an individual experiment. The mean of 5 experiments is represented by thick horizontal lines in each bottom panel. P values obtained with the paired Student t test are shown when significant; N.S. indicates a nonsignificant P value.

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