American Society of Hematology

Figure 2.

$Figure 2. HES-1 overexpression has no influence on myeloid differentiation but maintains CD34+ cells. Flow cytometric analysis of MS-5 cocultures with cytokine Mix 6 (SCF, FL3, TPO, IL-2, IL-7, and IL-15). Percentages of CD4+CD14+ monocytes (A) and CD45+CD34+ cells (B) in the EGFP+-gated fraction of cultures initiated with LIE-, HES-1-, or ICN-transduced CD34+ cord-blood cells are indicated. Dot plots shown are representative of 5 to 6 independent experiments with cells from 5 to 6 different donors. Scatterplots show relative and absolute numbers of monocytes and CD34+ cells generated in 5 to 6 independent experiments. Each symbol represents an individual experiment. The thick horizontal lines in each bottom panel represent the mean. P values obtained with the paired Student t test are shown when significant; N.S. indicates a nonsignificant P value.$

HES-1 overexpression has no influence on myeloid differentiation but maintains CD34⁺ cells. Flow cytometric analysis of MS-5 cocultures with cytokine Mix 6 (SCF, FL3, TPO, IL-2, IL-7, and IL-15). Percentages of CD4⁺CD14⁺ monocytes (A) and CD45⁺CD34⁺ cells (B) in the EGFP⁺-gated fraction of cultures initiated with LIE-, HES-1-, or ICN-transduced CD34⁺ cord-blood cells are indicated. Dot plots shown are representative of 5 to 6 independent experiments with cells from 5 to 6 different donors. Scatterplots show relative and absolute numbers of monocytes and CD34⁺ cells generated in 5 to 6 independent experiments. Each symbol represents an individual experiment. The thick horizontal lines in each bottom panel represent the mean. P values obtained with the paired Student t test are shown when significant; N.S. indicates a nonsignificant P value.

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