Induction of LMP-1 by IL-10 in other BL lines. (A) Total cell extracts of hIL-10-treated (50 ng/mL, 48 hours) Mutu I cl.148 and Mutu III cl.99 were analyzed for EBNA-2 and LMP-1 expression by SDS-PAGE and immunoblotting, developed on the same membrane. (B) Immunoblot analysis of LMP-1, EBNA-2, and β-actin expression in total cell extracts of IL-10-treated Sal BL cells (50 ng/mL, 48 hours). (C) Total cell extracts of hIL-10-treated (50 ng/mL, 48 hours) DG75-Akata Neo^R clones 2-2 and 6 were analyzed for EBNA-2 and LMP-1 expression by SDS-PAGE and immunoblotting. (D) Immunoblot analysis of LMP-1 expression in total cell lysates of IL-10-treated Daudi and P3HR1 cells (50 ng/mL, 6 hours). (E) RT-PCR results of LMP-2A, EBNA-2, and GAPDH expression of untreated and IL-10-treated (50 ng/mL, 24 hours) Daudi and P3HR1 cells. cDNAs prepared from the LCL B95-8 and the EBV-negative Hodgkin lymphoma cell line KMH2 were used as positive and negative controls, respectively. The PCR products were visualized in ethidiumbromide-stained agarose gels. The first lane contains 3 μL of GeneRuler 50-bp DNA ladder (MBI, Fermentas, Vilnius, Lithuania).