Eap interferes with α_vβ₃-integrin–dependent cell function. (A) VEGF (20 ng/mL)–stimulated migration of HUVECs toward VN, FBG, or FN (each 5 μg/mL) is shown in the absence (▪) or presence of the blocking mAb against α_vβ₃-integrin, LM609 (for VN and FBG), the blocking mAb against β₁-integrin, 6S6 (for FN) (20 μg/mL; □), or in the presence of Eap (▦;20 μg/mL), or protein A (▧;20 μg/mL). Cell migration is expressed relative to control, which is represented as cell migration in the absence of any stimulus or competitor. Data are means ± SD of 4 experiments performed in triplicate. *P < .05 compared with VEGF-stimulated migration in the absence of competitor. ns indicates nonsignificant. (B-D) Binding of VN to immobilized α_vβ₃-integrin (B), binding of FBG to immobilized α_vβ₃-integrin (C), and binding of FN to immobilized α₅β₁-integrin(D) was performed in the absence (–; ▪) or presence of blocking mAb against α_vβ₃-integrin, LM609 (for VN and FBG), the blocking mAb against β₁-integrin, 6S6 (for FN) (20 μg/mL; □), or increasing concentrations of Eap (▦) as indicated. Specific binding is expressed as absorbance at 405 nm. Data are means ± SD (n = 3) of a typical experiment; similar results were observed in 3 separate experiments.