Binding of βig-h3 to imDCs and macrophages but not mDCs. Macrophages, imDCs, and mDCs were incubated with purified βig-h3 at 4°C. Bound βig-h3 was detected with a mouse anti-myc antibody followed by PE-labeled goat anti–mouse IgG. (A) The cells were washed and then analyzed by flow cytometry. Filled histograms are signals obtained with isotype IgG. Solid lines indicate signals obtained with the anti-myc antibody. (B) Similarly stained cells were washed and examined under a fluorescence microscope.