DBA/2 spleen cells induced sclerodoma-like chronic GVHD in a dose-dependent manner. (A) Sublethally irradiated (6.5 Gy [650 rad]) BALB/c recipients were injected with graded numbers (3.125 × 10⁶ to 100 × 10⁶) of DBA/2 spleen cells. The left panel shows percentage of mice without proteinuria and the right panel shows the percentage of survival. There were 10 mice in each group combined from 2 replicate experiments. (B) Tissue sections of skin and kidney from GVHD BALB/c recipients injected 30 days earlier with DBA/2 or B10D2 spleen cells (50 × 10⁶) and control recipients without injection. The top row shows hematoxylin and eosin (HE) staining of skin tissues. The second row shows anti-collagen I staining of skin tissue. The third row shows immunofluorescent staining of IgG immune complex deposition in frozen skin tissues. The bottom row shows immunofluorescent staining of IgG immune complex deposition in frozen kidney tissues. Arrows show IgG immune complex deposition in glomerular capillary loops. One representative is shown of 4 examined recipients in each group.