Donor T-cell subsets and B cells in recipients given different donor cells. (A-C) The blood mononuclear cells from recipients given whole DBA/2 donor spleen cells (whole SPL-Rec), CD4⁺ T-cell-depleted spleen cells (CD4^--SPL-Rec), or CD8⁺ T-cell-depleted spleen cells (CD8^--SPL-Rec) were stained with anti-TCRαβ, CD4, CD8, and CD5.1 (donor T-cell marker) on day 15 after cell injection. The gated TCRαβ⁺CD5.1⁺ donor T cells are shown as TCRαβ versus CD4 or CD8. The percentage of CD5.1⁺ T cells among blood mononuclear cells and the percentage of CD4⁺ and CD8⁺ T cells among CD5.1⁺ donor T cells are shown above the gating boxes. One representative is shown of 8 recipients in each group. (D) The blood mononuclear cells from recipients given whole DBA/2 donor spleen or B220⁺ B-cell-depleted spleen (B220^--SPL-Rec) were stained with anti-TCRαβ versus anti-B220 15 and 30 days after donor-cell injection. The TCRαβ⁺ T cells and B220⁺ B cells are gated, and the percentage of those cells among mononuclear cells is shown beside the gating boxes. One representative is shown of 8 recipients in each group.