Lymph-node homing phenotype of CD40-activated B cells. (A) Expression of lymph node homing receptors on CD40-B cells. First row: Expression by CD40-activated B cells after 14 days of culture; similar results were obtained at later time points. Second row: Expression by unstimulated B cells. Third row: Expression by immature monocyte-derived DCs. Fourth row: Expression by monocyte-derived DCs upon maturation with anti-CD40L/TNF-α. One representative experiment of at least 8 is shown. (B-C) Migration of CD40-B cells. Total number of CD40-B cells in bottom chamber of Costar transwell plate after 2-hour migration through a 5-μm filter. Migration was determined for different concentrations of chemoattractants: (B) Ligand of CXCR4: CXCL12. (C) Ligands of CCR7: CCL21 (□) and CCL19 (▪). Mean values are shown; at least 6 independent experiments were performed.