Predicted effects of the Y94P and L125P, Q126P mutations on the secondary structure of the PIAS3_82-132 segment. The secondary structure was predicted with PSIPRED. Confidence values (ranging from 0-9; 9 indicates highest confidence) are shown in bars along the PIAS3 sequence for the PIAS3_1-628 protein (), the segment PIAS3_82-132 (▪), and the mutants PIAS3_{82-132; Y94P} (□) and PIAS3_{82-132; L125P, Q126P} (). Predicted α helices and β strands are shown below the graph as tubes and arrows, respectively. Predictions are similar for PIAS3_1-628 and PIAS3_82-132, except for more confident location of a central β strand in the full sequence secondary structure prediction. Mutation(s) to proline reduce the confidence level of the helix prediction, both for the N- and C-terminal helix. This effect is somewhat more expressed at the N-terminus due to its initially weaker helical signal. This figure indicates that the N-terminal helix might be abolished by the Y94P mutation, while the C-terminal helix is significantly shortened by the L125P, Q126P double mutation.