Possible structures for the PIAS3_82-132 segment created by ROBETTA de novo modeling. Three representative models are shown: (A) the lowest energy model of the prediction runs; (B) low-energy model of largest cluster that contains lowest energy models; and (C) a model that includes a beta hairpin. The predicted elements of secondary structure (according to PSIPRED, Figure 5) are colored in red (helix) and yellow (strand). The positions that were mutated to proline (eg, Y94, L125, and Q126) are shown in sticks. Y94 points mostly into the core but is also accessible to interactions with protein partners. In contrast, Q126 is mostly exposed.