Clustering of cell surface FasL in lipid rafts. 293T cells were transfected with a full-length FasL or E-cadherin expression plasmid and stained either with isotype control, anti-FasL, or anti-E-cadherin antibody (green). Lipid rafts were detected by staining with cholera toxin β subunit (CTX, red). Lipid raft clustering was induced by incubation with anti-CTX antibody. Confocal microscopy images for FasL(A) and E-cadherin (B) as nonraft-localized negative control are shown. Colocalization between lipid rafts and FasL, respectively. E-cadherin was analyzed using Imaris software and is depicted in white. Original magnification × 1000. Bars indicate 5 μm. A typical experiment of 5 is shown.