American Society of Hematology

Figure 2.

$Figure 2. Partial localization of FasL in lipid rafts. (A) 293T cells were transfected with full-length FasL and lipid rafts were isolated using the sucrose gradient method. FasL and flotillin in the raft and nonraft fractions were detected by Western blot, GM1 by dot blot. A typical experiment of 6 is shown. (B) 293T cells were transfected with FasL as described and lipid rafts were isolated using the short method. FasL, flotillin, and tubulin were detected by Western blot. NR indicates nonraft fraction; R, raft fraction. A typical experiment of 20 is shown. (C) Human peripheral blood T-cell blasts were stimulated with PMA and ionomycin, and lipid rafts were isolated using the sucrose density method. FasL in the raft and nonraft fractions was detected by Western blot, GM1 by dot blot. A typical experiment of 3 is shown. (D) Detection of FasL, flotillin, and Zap-70 in nonraft (NR) and raft (R) fraction of human T-cell blasts using the short raft isolation method. A typical experiment of 3 is shown.$

Partial localization of FasL in lipid rafts. (A) 293T cells were transfected with full-length FasL and lipid rafts were isolated using the sucrose gradient method. FasL and flotillin in the raft and nonraft fractions were detected by Western blot, GM1 by dot blot. A typical experiment of 6 is shown. (B) 293T cells were transfected with FasL as described and lipid rafts were isolated using the short method. FasL, flotillin, and tubulin were detected by Western blot. NR indicates nonraft fraction; R, raft fraction. A typical experiment of 20 is shown. (C) Human peripheral blood T-cell blasts were stimulated with PMA and ionomycin, and lipid rafts were isolated using the sucrose density method. FasL in the raft and nonraft fractions was detected by Western blot, GM1 by dot blot. A typical experiment of 3 is shown. (D) Detection of FasL, flotillin, and Zap-70 in nonraft (NR) and raft (R) fraction of human T-cell blasts using the short raft isolation method. A typical experiment of 3 is shown.

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