Methyl-β-cyclodextrin extrudes FasL from lipid rafts. (A) FasL-transfected 293T cells were treated for 30 minutes with control or 10 mM MbCD and rested for 30 minutes in medium, and lipid rafts were isolated. FasL and flotillin were detected by Western blot. NR indicates nonraft fraction; R, raft fraction. Typical experiments of 5 are shown. (B) FasL-transfected 293T cells were treated with MbCD as described and FasL cell surface expression was detected by flow cytometry. A typical experiment of 3 is shown.